Electroporation of Mycobacteria

Electroporation of the Testis

The mature mammalian testis is a marvelous organ that produces numerous sperm cells during its reproductive phase. This biologically significant process consists of three steps: stem cell self-renewal and differentiation, meiosis and genetic recombination, and haploid cell morphogenesis into sperm (Russell et al., 1990). The first step provides a good model for investigating the molecular mecha...

Electroporation of Craniofacial Mesenchyme

Electroporation is an efficient method of delivering DNA and other charged macromolecules into tissues at precise time points and in precise locations. For example, electroporation has been used with great success to study neural and retinal development in Xenopus, chicken and mouse (1-10). However, it is important to note that in all of these studies, investigators were not targeting soft tiss...

Electroporation of the heart.

Defibrillation shocks are commonly used to terminate life-threatening arrhythmias. According to the excitation theory of defibrillation, such shocks are aimed at depolarizing the membranes of most cardiac cells resulting in resynchronization of electrical activity in the heart. If shock-induced changes in transmembrane potential are large enough, they can cause transient tissue damage due to el...

Electroporation of Biological Membranes

In recent years, manipulation of biological cells and cell tissue by external electric fields gains increasing importance for biophysics and cell biology in general and in biotechnology and medicine in particular. Especially, the method of electroporation has become a powerfool tool for cell manipulations. In electroporation cells are subjected to a pulsed high-voltage electric field, resulting...

Intermediary metabolism of mycobacteria.